human t lymphocyte cell line jurkat t cell Search Results


tetracycline inducible trextm jurkat cells  (Thermo Fisher)
99
Thermo Fisher tetracycline inducible trextm jurkat cells
Tetracycline Inducible Trextm Jurkat Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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manganese sod mnsod jurkat cells  (Thermo Fisher)
98
Thermo Fisher manganese sod mnsod jurkat cells
Manganese Sod Mnsod Jurkat Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kyse150  (ATCC)
99
ATCC kyse150
a Three-dimensional visualization of α-tubulin and Fn in E109 cells. The cells were infected with Fn (MOI of 1:10) for 48 h. Scale bar: 50 μm (left). b , c Intracellular bacterial proliferation was assessed by a gentamycin protection assay. E109, <t>Kyse150</t> and AKR cells were lysed at the indicated time points after Fn (MOI of 1:10) infection, and the numbers of total viable bacteria ( b ) and viable bacteria per ESCC cell ( c ) were determined by the serial dilution method. P means vs . the 24 h group. d , e FACS of PD-L1 + membrane expression in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:10) for 48 h and quantification. f , g Dichromatic IF staining of PD-L1 and Fn in E109, Kyse150 and AKR cells. The cells were infected with Fn (MOI of 1:10) for 48 h. f Representative images. Scale bar: 20 μm. g Quantification. h qRT‒PCR analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:1, 1:10, 1:100) for 48 h or infection with Fn (MOI of 1:10) for 24, 48 or 72 h. P means vs . the Con group. i , j Immunoblotting analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn ( i ) and quantification ( j ). P means vs . the Con group. k Immunoblotting analysis and quantification of PD-L1 in tumors from C57BL/6 xenografts (mean ± SD; the experiment was done once; n = 3 mice per group). l IF staining of PD-L1 or Fn in tumor tissues from C57BL/6 xenografts. Scale bar: 50 μm. Images in a and l were representative results of n = 3 independent experiments with similar results. Results in b , c , e , g , h and j were presented as n = 3 biological replicates, mean ± SD, ns means not significant. The statistical significance of results in b , c , h , j and k were determined by one-way ANOVA analysis. e and g were determined by a two-tailed unpaired t -test.
Kyse150, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+t+lymphocyte+cell+line+jurkat+t+cell/Jurkat%2C+Clone+E6-1/pmc10507087-292-8-22
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human cd4 t cell line jurkat  (ATCC)
95
ATCC human cd4 t cell line jurkat
a Three-dimensional visualization of α-tubulin and Fn in E109 cells. The cells were infected with Fn (MOI of 1:10) for 48 h. Scale bar: 50 μm (left). b , c Intracellular bacterial proliferation was assessed by a gentamycin protection assay. E109, <t>Kyse150</t> and AKR cells were lysed at the indicated time points after Fn (MOI of 1:10) infection, and the numbers of total viable bacteria ( b ) and viable bacteria per ESCC cell ( c ) were determined by the serial dilution method. P means vs . the 24 h group. d , e FACS of PD-L1 + membrane expression in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:10) for 48 h and quantification. f , g Dichromatic IF staining of PD-L1 and Fn in E109, Kyse150 and AKR cells. The cells were infected with Fn (MOI of 1:10) for 48 h. f Representative images. Scale bar: 20 μm. g Quantification. h qRT‒PCR analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:1, 1:10, 1:100) for 48 h or infection with Fn (MOI of 1:10) for 24, 48 or 72 h. P means vs . the Con group. i , j Immunoblotting analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn ( i ) and quantification ( j ). P means vs . the Con group. k Immunoblotting analysis and quantification of PD-L1 in tumors from C57BL/6 xenografts (mean ± SD; the experiment was done once; n = 3 mice per group). l IF staining of PD-L1 or Fn in tumor tissues from C57BL/6 xenografts. Scale bar: 50 μm. Images in a and l were representative results of n = 3 independent experiments with similar results. Results in b , c , e , g , h and j were presented as n = 3 biological replicates, mean ± SD, ns means not significant. The statistical significance of results in b , c , h , j and k were determined by one-way ANOVA analysis. e and g were determined by a two-tailed unpaired t -test.
Human Cd4 T Cell Line Jurkat, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rrid cvcl 0367 293t cells takara 632180  (ATCC)
99
ATCC rrid cvcl 0367 293t cells takara 632180
a Three-dimensional visualization of α-tubulin and Fn in E109 cells. The cells were infected with Fn (MOI of 1:10) for 48 h. Scale bar: 50 μm (left). b , c Intracellular bacterial proliferation was assessed by a gentamycin protection assay. E109, <t>Kyse150</t> and AKR cells were lysed at the indicated time points after Fn (MOI of 1:10) infection, and the numbers of total viable bacteria ( b ) and viable bacteria per ESCC cell ( c ) were determined by the serial dilution method. P means vs . the 24 h group. d , e FACS of PD-L1 + membrane expression in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:10) for 48 h and quantification. f , g Dichromatic IF staining of PD-L1 and Fn in E109, Kyse150 and AKR cells. The cells were infected with Fn (MOI of 1:10) for 48 h. f Representative images. Scale bar: 20 μm. g Quantification. h qRT‒PCR analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:1, 1:10, 1:100) for 48 h or infection with Fn (MOI of 1:10) for 24, 48 or 72 h. P means vs . the Con group. i , j Immunoblotting analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn ( i ) and quantification ( j ). P means vs . the Con group. k Immunoblotting analysis and quantification of PD-L1 in tumors from C57BL/6 xenografts (mean ± SD; the experiment was done once; n = 3 mice per group). l IF staining of PD-L1 or Fn in tumor tissues from C57BL/6 xenografts. Scale bar: 50 μm. Images in a and l were representative results of n = 3 independent experiments with similar results. Results in b , c , e , g , h and j were presented as n = 3 biological replicates, mean ± SD, ns means not significant. The statistical significance of results in b , c , h , j and k were determined by one-way ANOVA analysis. e and g were determined by a two-tailed unpaired t -test.
Rrid Cvcl 0367 293t Cells Takara 632180, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jurkat cells  (ATCC)
94
ATCC jurkat cells
a Three-dimensional visualization of α-tubulin and Fn in E109 cells. The cells were infected with Fn (MOI of 1:10) for 48 h. Scale bar: 50 μm (left). b , c Intracellular bacterial proliferation was assessed by a gentamycin protection assay. E109, <t>Kyse150</t> and AKR cells were lysed at the indicated time points after Fn (MOI of 1:10) infection, and the numbers of total viable bacteria ( b ) and viable bacteria per ESCC cell ( c ) were determined by the serial dilution method. P means vs . the 24 h group. d , e FACS of PD-L1 + membrane expression in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:10) for 48 h and quantification. f , g Dichromatic IF staining of PD-L1 and Fn in E109, Kyse150 and AKR cells. The cells were infected with Fn (MOI of 1:10) for 48 h. f Representative images. Scale bar: 20 μm. g Quantification. h qRT‒PCR analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:1, 1:10, 1:100) for 48 h or infection with Fn (MOI of 1:10) for 24, 48 or 72 h. P means vs . the Con group. i , j Immunoblotting analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn ( i ) and quantification ( j ). P means vs . the Con group. k Immunoblotting analysis and quantification of PD-L1 in tumors from C57BL/6 xenografts (mean ± SD; the experiment was done once; n = 3 mice per group). l IF staining of PD-L1 or Fn in tumor tissues from C57BL/6 xenografts. Scale bar: 50 μm. Images in a and l were representative results of n = 3 independent experiments with similar results. Results in b , c , e , g , h and j were presented as n = 3 biological replicates, mean ± SD, ns means not significant. The statistical significance of results in b , c , h , j and k were determined by one-way ANOVA analysis. e and g were determined by a two-tailed unpaired t -test.
Jurkat Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+t+lymphocyte+cell+line+jurkat+t+cell/Neo+Jurkat/pmc05500420-128-10-28
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human bcl2  (ATCC)
99
ATCC human bcl2
a Three-dimensional visualization of α-tubulin and Fn in E109 cells. The cells were infected with Fn (MOI of 1:10) for 48 h. Scale bar: 50 μm (left). b , c Intracellular bacterial proliferation was assessed by a gentamycin protection assay. E109, <t>Kyse150</t> and AKR cells were lysed at the indicated time points after Fn (MOI of 1:10) infection, and the numbers of total viable bacteria ( b ) and viable bacteria per ESCC cell ( c ) were determined by the serial dilution method. P means vs . the 24 h group. d , e FACS of PD-L1 + membrane expression in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:10) for 48 h and quantification. f , g Dichromatic IF staining of PD-L1 and Fn in E109, Kyse150 and AKR cells. The cells were infected with Fn (MOI of 1:10) for 48 h. f Representative images. Scale bar: 20 μm. g Quantification. h qRT‒PCR analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:1, 1:10, 1:100) for 48 h or infection with Fn (MOI of 1:10) for 24, 48 or 72 h. P means vs . the Con group. i , j Immunoblotting analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn ( i ) and quantification ( j ). P means vs . the Con group. k Immunoblotting analysis and quantification of PD-L1 in tumors from C57BL/6 xenografts (mean ± SD; the experiment was done once; n = 3 mice per group). l IF staining of PD-L1 or Fn in tumor tissues from C57BL/6 xenografts. Scale bar: 50 μm. Images in a and l were representative results of n = 3 independent experiments with similar results. Results in b , c , e , g , h and j were presented as n = 3 biological replicates, mean ± SD, ns means not significant. The statistical significance of results in b , c , h , j and k were determined by one-way ANOVA analysis. e and g were determined by a two-tailed unpaired t -test.
Human Bcl2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+t+lymphocyte+cell+line+jurkat+t+cell/BCL2+Jurkat/10__1158_slash_1535___7163__mct___17___0099-48-19-28
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t lymphoblastic lymphoma leukemia atcc  (ATCC)
92
ATCC t lymphoblastic lymphoma leukemia atcc
a Three-dimensional visualization of α-tubulin and Fn in E109 cells. The cells were infected with Fn (MOI of 1:10) for 48 h. Scale bar: 50 μm (left). b , c Intracellular bacterial proliferation was assessed by a gentamycin protection assay. E109, <t>Kyse150</t> and AKR cells were lysed at the indicated time points after Fn (MOI of 1:10) infection, and the numbers of total viable bacteria ( b ) and viable bacteria per ESCC cell ( c ) were determined by the serial dilution method. P means vs . the 24 h group. d , e FACS of PD-L1 + membrane expression in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:10) for 48 h and quantification. f , g Dichromatic IF staining of PD-L1 and Fn in E109, Kyse150 and AKR cells. The cells were infected with Fn (MOI of 1:10) for 48 h. f Representative images. Scale bar: 20 μm. g Quantification. h qRT‒PCR analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:1, 1:10, 1:100) for 48 h or infection with Fn (MOI of 1:10) for 24, 48 or 72 h. P means vs . the Con group. i , j Immunoblotting analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn ( i ) and quantification ( j ). P means vs . the Con group. k Immunoblotting analysis and quantification of PD-L1 in tumors from C57BL/6 xenografts (mean ± SD; the experiment was done once; n = 3 mice per group). l IF staining of PD-L1 or Fn in tumor tissues from C57BL/6 xenografts. Scale bar: 50 μm. Images in a and l were representative results of n = 3 independent experiments with similar results. Results in b , c , e , g , h and j were presented as n = 3 biological replicates, mean ± SD, ns means not significant. The statistical significance of results in b , c , h , j and k were determined by one-way ANOVA analysis. e and g were determined by a two-tailed unpaired t -test.
T Lymphoblastic Lymphoma Leukemia Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+t+lymphocyte+cell+line+jurkat+t+cell/H33HJ-JA1+Human+Lymphoma%2C+Jurkat+Derivative/10__1007_slash_bf03401961-54-122-124
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c human leukemia jurkat t cells  (ATCC)
95
ATCC c human leukemia jurkat t cells
a Three-dimensional visualization of α-tubulin and Fn in E109 cells. The cells were infected with Fn (MOI of 1:10) for 48 h. Scale bar: 50 μm (left). b , c Intracellular bacterial proliferation was assessed by a gentamycin protection assay. E109, <t>Kyse150</t> and AKR cells were lysed at the indicated time points after Fn (MOI of 1:10) infection, and the numbers of total viable bacteria ( b ) and viable bacteria per ESCC cell ( c ) were determined by the serial dilution method. P means vs . the 24 h group. d , e FACS of PD-L1 + membrane expression in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:10) for 48 h and quantification. f , g Dichromatic IF staining of PD-L1 and Fn in E109, Kyse150 and AKR cells. The cells were infected with Fn (MOI of 1:10) for 48 h. f Representative images. Scale bar: 20 μm. g Quantification. h qRT‒PCR analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:1, 1:10, 1:100) for 48 h or infection with Fn (MOI of 1:10) for 24, 48 or 72 h. P means vs . the Con group. i , j Immunoblotting analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn ( i ) and quantification ( j ). P means vs . the Con group. k Immunoblotting analysis and quantification of PD-L1 in tumors from C57BL/6 xenografts (mean ± SD; the experiment was done once; n = 3 mice per group). l IF staining of PD-L1 or Fn in tumor tissues from C57BL/6 xenografts. Scale bar: 50 μm. Images in a and l were representative results of n = 3 independent experiments with similar results. Results in b , c , e , g , h and j were presented as n = 3 biological replicates, mean ± SD, ns means not significant. The statistical significance of results in b , c , h , j and k were determined by one-way ANOVA analysis. e and g were determined by a two-tailed unpaired t -test.
C Human Leukemia Jurkat T Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c human leukemia jurkat t cells - by Bioz Stars, 2026-09
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jurkat cells  (National Centre for Cell Science)
90
National Centre for Cell Science jurkat cells
Schematic representation of experimental set up. (A) To mimic the effect of acidic pH on immune cells, <t>Jurkat</t> cells were exposed to different pH conditions, pH 7.4, pH 6.9, and pH 6.5, for 24 h. Total mRNA was isolated for further experiments. (B) To ascertain the effect of cancer cells in the acidic environment on immune cells, Jurkat cells were cultured in HCM of pH 7.4, pH 6.9, and pH 6.5 for 24 h. Further, total mRNA was isolated from Jurkat cells for further experiments. Similar studies were performed for THP-1 also. <t>HCM,</t> <t>HeLa</t> cells conditioned medium.
Jurkat Cells, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+t+lymphocyte+cell+line+jurkat+t+cell/jurkat/pmc10505064-15-7-21
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human leukaemia jurkat  (BioResource International Inc)
90
BioResource International Inc human leukaemia jurkat
Schematic representation of experimental set up. (A) To mimic the effect of acidic pH on immune cells, <t>Jurkat</t> cells were exposed to different pH conditions, pH 7.4, pH 6.9, and pH 6.5, for 24 h. Total mRNA was isolated for further experiments. (B) To ascertain the effect of cancer cells in the acidic environment on immune cells, Jurkat cells were cultured in HCM of pH 7.4, pH 6.9, and pH 6.5 for 24 h. Further, total mRNA was isolated from Jurkat cells for further experiments. Similar studies were performed for THP-1 also. <t>HCM,</t> <t>HeLa</t> cells conditioned medium.
Human Leukaemia Jurkat, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jurkat t cells  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology jurkat t cells
Schematic representation of experimental set up. (A) To mimic the effect of acidic pH on immune cells, <t>Jurkat</t> cells were exposed to different pH conditions, pH 7.4, pH 6.9, and pH 6.5, for 24 h. Total mRNA was isolated for further experiments. (B) To ascertain the effect of cancer cells in the acidic environment on immune cells, Jurkat cells were cultured in HCM of pH 7.4, pH 6.9, and pH 6.5 for 24 h. Further, total mRNA was isolated from Jurkat cells for further experiments. Similar studies were performed for THP-1 also. <t>HCM,</t> <t>HeLa</t> cells conditioned medium.
Jurkat T Cells, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a Three-dimensional visualization of α-tubulin and Fn in E109 cells. The cells were infected with Fn (MOI of 1:10) for 48 h. Scale bar: 50 μm (left). b , c Intracellular bacterial proliferation was assessed by a gentamycin protection assay. E109, Kyse150 and AKR cells were lysed at the indicated time points after Fn (MOI of 1:10) infection, and the numbers of total viable bacteria ( b ) and viable bacteria per ESCC cell ( c ) were determined by the serial dilution method. P means vs . the 24 h group. d , e FACS of PD-L1 + membrane expression in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:10) for 48 h and quantification. f , g Dichromatic IF staining of PD-L1 and Fn in E109, Kyse150 and AKR cells. The cells were infected with Fn (MOI of 1:10) for 48 h. f Representative images. Scale bar: 20 μm. g Quantification. h qRT‒PCR analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:1, 1:10, 1:100) for 48 h or infection with Fn (MOI of 1:10) for 24, 48 or 72 h. P means vs . the Con group. i , j Immunoblotting analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn ( i ) and quantification ( j ). P means vs . the Con group. k Immunoblotting analysis and quantification of PD-L1 in tumors from C57BL/6 xenografts (mean ± SD; the experiment was done once; n = 3 mice per group). l IF staining of PD-L1 or Fn in tumor tissues from C57BL/6 xenografts. Scale bar: 50 μm. Images in a and l were representative results of n = 3 independent experiments with similar results. Results in b , c , e , g , h and j were presented as n = 3 biological replicates, mean ± SD, ns means not significant. The statistical significance of results in b , c , h , j and k were determined by one-way ANOVA analysis. e and g were determined by a two-tailed unpaired t -test.

Journal: Nature Communications

Article Title: Intracellular Fusobacterium nucleatum infection attenuates antitumor immunity in esophageal squamous cell carcinoma

doi: 10.1038/s41467-023-40987-3

Figure Lengend Snippet: a Three-dimensional visualization of α-tubulin and Fn in E109 cells. The cells were infected with Fn (MOI of 1:10) for 48 h. Scale bar: 50 μm (left). b , c Intracellular bacterial proliferation was assessed by a gentamycin protection assay. E109, Kyse150 and AKR cells were lysed at the indicated time points after Fn (MOI of 1:10) infection, and the numbers of total viable bacteria ( b ) and viable bacteria per ESCC cell ( c ) were determined by the serial dilution method. P means vs . the 24 h group. d , e FACS of PD-L1 + membrane expression in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:10) for 48 h and quantification. f , g Dichromatic IF staining of PD-L1 and Fn in E109, Kyse150 and AKR cells. The cells were infected with Fn (MOI of 1:10) for 48 h. f Representative images. Scale bar: 20 μm. g Quantification. h qRT‒PCR analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn (MOI of 1:1, 1:10, 1:100) for 48 h or infection with Fn (MOI of 1:10) for 24, 48 or 72 h. P means vs . the Con group. i , j Immunoblotting analysis of PD-L1 in E109, Kyse150 and AKR cells after infection with Fn ( i ) and quantification ( j ). P means vs . the Con group. k Immunoblotting analysis and quantification of PD-L1 in tumors from C57BL/6 xenografts (mean ± SD; the experiment was done once; n = 3 mice per group). l IF staining of PD-L1 or Fn in tumor tissues from C57BL/6 xenografts. Scale bar: 50 μm. Images in a and l were representative results of n = 3 independent experiments with similar results. Results in b , c , e , g , h and j were presented as n = 3 biological replicates, mean ± SD, ns means not significant. The statistical significance of results in b , c , h , j and k were determined by one-way ANOVA analysis. e and g were determined by a two-tailed unpaired t -test.

Article Snippet: The human ESCC cell line Eca109 (E109) and Kyse150 (a kind gift from professor Musheng Zeng, SYSUCC, Guangzhou, China), Jurkat cell line (ATCC TIB-152), embryonic kidney 293 T cells (ATCC CRL-3216), and mouse ESCC cell line AKR (C945, Wuhan Sunncell Biotechnology, China) were cultured in RPMI 1640 or DMEM medium (Gibco, CA, USA), respectively.

Techniques: Infection, Bacteria, Serial Dilution, Membrane, Expressing, Staining, Western Blot, Two Tailed Test

a – c Dichromatic IF staining of Fn and Fn-Dps ( a ), scale bar: 40 μm (top). Immunoblotting ( b ) and FACS ( c ) analysis of PD-L1. Cells were infected with Fn (MOI of 1:10) or treated with Fn-Dps (1 μM) for 48 h. d – f Venn diagram showing the unique and overlapping predicted transcription factors in different groups. The blue dots represent differentially expressed transcription factor genes (POU6F1, CEBPB, NR4A2 and MAFG). qRT‒PCR analysis ( f ). g Analysis of the relevance of the CD274 (PD-L1) and ATF3 genes in ESCA tissues (TCGA database, n = 173). h – j ATF3 and PD-L1 expression was analyzed by western blotting. ESCC cells were transfected with ATF3 overexpression (OE) or negative control (CTRL) vectors for 48 h ( h ). Co-IP assay ( i ). Cells were treated with Fn-Dps (1 μM) for 48 h after transfection with siRNA against ATF3 for 48 h ( j ). k , l Representative images of IHC staining ( k ) and IF staining quantification ( l ) of ATF3 or PD-L1 expression in tumors from C57BL/6 xenografts. Scale bar: 10 μm. m The interaction of Fn-Dps with ATF3 in ESCC cells after treated with Fn-Dps (1 μM) for 48 h was detected by Co-IP assay. n Analysis of CD274 WT or mutant promoter activity in 293 T cells transfected with ATF3-Flag and treated with Fn-Dps (1 μM) for 48 h. o ChIP‒qPCR analysis of the relative enrichment of ATF3 at the CD274 gene promoter in Kyse150 cells. p , q Dichromatic IF staining of ATF3 and Fn-Dps (p), scale bar: 50 μm (top). Immunoblotting of nuclear (N) and cytoplasmic (C) ( q ). Cells were treated with Fn-Dps (1 μM) for 48 h. Images in a , h , j , m , p and q were representative results of n = 3 independent experiments with similar results. Results in b , c , n , and o were presented as n = 3 biological replicates, mean ± SD. Statistical significance in b , c was determined by a two-tailed unpaired t -test. l , n and o were determined by two-way ANOVA multiple comparisons. g was determined by two-tailed nonparametric Spearman correlation analysis.

Journal: Nature Communications

Article Title: Intracellular Fusobacterium nucleatum infection attenuates antitumor immunity in esophageal squamous cell carcinoma

doi: 10.1038/s41467-023-40987-3

Figure Lengend Snippet: a – c Dichromatic IF staining of Fn and Fn-Dps ( a ), scale bar: 40 μm (top). Immunoblotting ( b ) and FACS ( c ) analysis of PD-L1. Cells were infected with Fn (MOI of 1:10) or treated with Fn-Dps (1 μM) for 48 h. d – f Venn diagram showing the unique and overlapping predicted transcription factors in different groups. The blue dots represent differentially expressed transcription factor genes (POU6F1, CEBPB, NR4A2 and MAFG). qRT‒PCR analysis ( f ). g Analysis of the relevance of the CD274 (PD-L1) and ATF3 genes in ESCA tissues (TCGA database, n = 173). h – j ATF3 and PD-L1 expression was analyzed by western blotting. ESCC cells were transfected with ATF3 overexpression (OE) or negative control (CTRL) vectors for 48 h ( h ). Co-IP assay ( i ). Cells were treated with Fn-Dps (1 μM) for 48 h after transfection with siRNA against ATF3 for 48 h ( j ). k , l Representative images of IHC staining ( k ) and IF staining quantification ( l ) of ATF3 or PD-L1 expression in tumors from C57BL/6 xenografts. Scale bar: 10 μm. m The interaction of Fn-Dps with ATF3 in ESCC cells after treated with Fn-Dps (1 μM) for 48 h was detected by Co-IP assay. n Analysis of CD274 WT or mutant promoter activity in 293 T cells transfected with ATF3-Flag and treated with Fn-Dps (1 μM) for 48 h. o ChIP‒qPCR analysis of the relative enrichment of ATF3 at the CD274 gene promoter in Kyse150 cells. p , q Dichromatic IF staining of ATF3 and Fn-Dps (p), scale bar: 50 μm (top). Immunoblotting of nuclear (N) and cytoplasmic (C) ( q ). Cells were treated with Fn-Dps (1 μM) for 48 h. Images in a , h , j , m , p and q were representative results of n = 3 independent experiments with similar results. Results in b , c , n , and o were presented as n = 3 biological replicates, mean ± SD. Statistical significance in b , c was determined by a two-tailed unpaired t -test. l , n and o were determined by two-way ANOVA multiple comparisons. g was determined by two-tailed nonparametric Spearman correlation analysis.

Article Snippet: The human ESCC cell line Eca109 (E109) and Kyse150 (a kind gift from professor Musheng Zeng, SYSUCC, Guangzhou, China), Jurkat cell line (ATCC TIB-152), embryonic kidney 293 T cells (ATCC CRL-3216), and mouse ESCC cell line AKR (C945, Wuhan Sunncell Biotechnology, China) were cultured in RPMI 1640 or DMEM medium (Gibco, CA, USA), respectively.

Techniques: Staining, Western Blot, Infection, Expressing, Transfection, Over Expression, Negative Control, Co-Immunoprecipitation Assay, Immunohistochemistry, Mutagenesis, Activity Assay, Two Tailed Test

Schematic representation of experimental set up. (A) To mimic the effect of acidic pH on immune cells, Jurkat cells were exposed to different pH conditions, pH 7.4, pH 6.9, and pH 6.5, for 24 h. Total mRNA was isolated for further experiments. (B) To ascertain the effect of cancer cells in the acidic environment on immune cells, Jurkat cells were cultured in HCM of pH 7.4, pH 6.9, and pH 6.5 for 24 h. Further, total mRNA was isolated from Jurkat cells for further experiments. Similar studies were performed for THP-1 also. HCM, HeLa cells conditioned medium.

Journal: Asian Biomedicine: Research, Reviews and News

Article Title: Acidic environment could modulate the interferon-γ expression: Implication on modulation of cancer and immune cells’ interactions

doi: 10.2478/abm-2023-0047

Figure Lengend Snippet: Schematic representation of experimental set up. (A) To mimic the effect of acidic pH on immune cells, Jurkat cells were exposed to different pH conditions, pH 7.4, pH 6.9, and pH 6.5, for 24 h. Total mRNA was isolated for further experiments. (B) To ascertain the effect of cancer cells in the acidic environment on immune cells, Jurkat cells were cultured in HCM of pH 7.4, pH 6.9, and pH 6.5 for 24 h. Further, total mRNA was isolated from Jurkat cells for further experiments. Similar studies were performed for THP-1 also. HCM, HeLa cells conditioned medium.

Article Snippet: Exponentially growing HeLa cells (human cervical cancer), Jurkat cells (human T cell lymphoma), and THP-1 cells (monocytes) were procured from the National Centre for Cell Science (NCCS), India.

Techniques: Isolation, Cell Culture

Schematic representation of experimental setup to ascertain the effect of the acidic environment in the restriction of immune response. Jurkat cells were stimulated with HCM at pH 7.4, pH 6.9, and pH 6.5 for 20 h. Cell-free media thus obtained were referred to as CM1 and saved for cytotoxic assessment against the HeLa cells. Jurkat cells obtained from this step were further subjected to reversal of pH by culturing the cells in a fresh medium of pH 7.4 for 4 h. After this, the media were extracted, referred to as CM2, and saved for cytotoxic assessment against the HeLa cells. A similar experiment was also carried out for THP-1. HCM, HeLa cells conditioned medium.

Journal: Asian Biomedicine: Research, Reviews and News

Article Title: Acidic environment could modulate the interferon-γ expression: Implication on modulation of cancer and immune cells’ interactions

doi: 10.2478/abm-2023-0047

Figure Lengend Snippet: Schematic representation of experimental setup to ascertain the effect of the acidic environment in the restriction of immune response. Jurkat cells were stimulated with HCM at pH 7.4, pH 6.9, and pH 6.5 for 20 h. Cell-free media thus obtained were referred to as CM1 and saved for cytotoxic assessment against the HeLa cells. Jurkat cells obtained from this step were further subjected to reversal of pH by culturing the cells in a fresh medium of pH 7.4 for 4 h. After this, the media were extracted, referred to as CM2, and saved for cytotoxic assessment against the HeLa cells. A similar experiment was also carried out for THP-1. HCM, HeLa cells conditioned medium.

Article Snippet: Exponentially growing HeLa cells (human cervical cancer), Jurkat cells (human T cell lymphoma), and THP-1 cells (monocytes) were procured from the National Centre for Cell Science (NCCS), India.

Techniques:

Expression of IFN-γ under physiological pH (pH 7.4) and acidic environment (pH 6.9, pH 6.5) in Jurkat (A, C, E) and THP-1 (B, D, F) cells after 24 h incubation. (A, B) Semi-quantitative PCR of IFN-γ mRNA expression under physiological pH (pH 7.4) and acidic environment (pH 6.9, pH 6.5). Representative agarose gel of PCR amplified products from 1 of 3 independent similar experiments. ( C, D ) Quantitative real-time PCR analysis of IFN-γ mRNA expression under physiological pH (pH 7.4) and acidic environment (pH 6.9, pH 6.5). The delta CT method was used for relative quantification. For relative quantification, cells grown at pH 7.4 were taken as control. Data were normalized to β-actin. The specificity of reaction products was analyzed by melting curve analysis. The bar graph indicated the fold change in the expression of IFN-γ. (E, F) Representative histogram of flow cytometric analysis of anti–IFN-γ antibody stained Jurkat cells ( E ) and THP-1 cells ( F ). Mean fluorescence depicted the level of IFN-γ. Ten thousand events were counted per tube. Data are representative of 1 of 3 independent similar experiments. * P < 0.05. IFN-γ, interferon gamma.

Journal: Asian Biomedicine: Research, Reviews and News

Article Title: Acidic environment could modulate the interferon-γ expression: Implication on modulation of cancer and immune cells’ interactions

doi: 10.2478/abm-2023-0047

Figure Lengend Snippet: Expression of IFN-γ under physiological pH (pH 7.4) and acidic environment (pH 6.9, pH 6.5) in Jurkat (A, C, E) and THP-1 (B, D, F) cells after 24 h incubation. (A, B) Semi-quantitative PCR of IFN-γ mRNA expression under physiological pH (pH 7.4) and acidic environment (pH 6.9, pH 6.5). Representative agarose gel of PCR amplified products from 1 of 3 independent similar experiments. ( C, D ) Quantitative real-time PCR analysis of IFN-γ mRNA expression under physiological pH (pH 7.4) and acidic environment (pH 6.9, pH 6.5). The delta CT method was used for relative quantification. For relative quantification, cells grown at pH 7.4 were taken as control. Data were normalized to β-actin. The specificity of reaction products was analyzed by melting curve analysis. The bar graph indicated the fold change in the expression of IFN-γ. (E, F) Representative histogram of flow cytometric analysis of anti–IFN-γ antibody stained Jurkat cells ( E ) and THP-1 cells ( F ). Mean fluorescence depicted the level of IFN-γ. Ten thousand events were counted per tube. Data are representative of 1 of 3 independent similar experiments. * P < 0.05. IFN-γ, interferon gamma.

Article Snippet: Exponentially growing HeLa cells (human cervical cancer), Jurkat cells (human T cell lymphoma), and THP-1 cells (monocytes) were procured from the National Centre for Cell Science (NCCS), India.

Techniques: Expressing, Incubation, Real-time Polymerase Chain Reaction, Agarose Gel Electrophoresis, Amplification, Quantitative Proteomics, Control, Staining, Fluorescence

Expression of IFN-γ in Jurkat (A, C, E) and THP-1 (B, D, F) cells cultured in HCM at different pH (pH 7.4, pH 6.9, and pH 6.5). (A, B) Semi-quantitative PCR of IFN-γ mRNA expression in Jurkat and THP-1 cells cultured in HCM at pH 7.4, pH 6.9, and pH 6.5. Representative agarose gel of PCR amplified product from 1 of 3 independent similar experiments. (C, D) Quantitative real-time PCR analysis of altered IFN-γ mRNA level in Jurkat and THP-1 cells cultured in HCM at pH 7.4, pH 6.9, pH 6.5. For relative quantification, cells grown in HCM at pH 7.4 were taken as control. Data was normalized to β-actin. The specificity of the reaction products was analyzed by melting curve analysis (B) Representative histogram of flow cytometric analysis of IFN-γ stained Jurkat (C) and THP-1 (D) cells after treatment. Mean fluorescence depicted the level of IFN-γ. Ten thousand events were counted per tube. Data are representative of 1 of 3 independent similar experiments. HCM, HeLa cells conditioned medium; IFN-γ, interferon gamma.

Journal: Asian Biomedicine: Research, Reviews and News

Article Title: Acidic environment could modulate the interferon-γ expression: Implication on modulation of cancer and immune cells’ interactions

doi: 10.2478/abm-2023-0047

Figure Lengend Snippet: Expression of IFN-γ in Jurkat (A, C, E) and THP-1 (B, D, F) cells cultured in HCM at different pH (pH 7.4, pH 6.9, and pH 6.5). (A, B) Semi-quantitative PCR of IFN-γ mRNA expression in Jurkat and THP-1 cells cultured in HCM at pH 7.4, pH 6.9, and pH 6.5. Representative agarose gel of PCR amplified product from 1 of 3 independent similar experiments. (C, D) Quantitative real-time PCR analysis of altered IFN-γ mRNA level in Jurkat and THP-1 cells cultured in HCM at pH 7.4, pH 6.9, pH 6.5. For relative quantification, cells grown in HCM at pH 7.4 were taken as control. Data was normalized to β-actin. The specificity of the reaction products was analyzed by melting curve analysis (B) Representative histogram of flow cytometric analysis of IFN-γ stained Jurkat (C) and THP-1 (D) cells after treatment. Mean fluorescence depicted the level of IFN-γ. Ten thousand events were counted per tube. Data are representative of 1 of 3 independent similar experiments. HCM, HeLa cells conditioned medium; IFN-γ, interferon gamma.

Article Snippet: Exponentially growing HeLa cells (human cervical cancer), Jurkat cells (human T cell lymphoma), and THP-1 cells (monocytes) were procured from the National Centre for Cell Science (NCCS), India.

Techniques: Expressing, Cell Culture, Real-time Polymerase Chain Reaction, Agarose Gel Electrophoresis, Amplification, Quantitative Proteomics, Control, Staining, Fluorescence

Altered relative expression of IFN-γ in Jurkat cells and THP-1 cells with and without stimulation with HCM at different pH values of 7.4, 6.9, and 6.5. A quantitative real-time PCR based comparison of IFN-γ produced by Jurkat cells (A) and THP-1 (B) cultured at different pH values (7.4, 6.9, and 6.5) and HCM at different pH (HCM pH 7.4, HCM pH 6.9, and HCM pH 6.5). * P < 0.05. HCM, HeLa cells conditioned medium; IFN-γ, interferon gamma.

Journal: Asian Biomedicine: Research, Reviews and News

Article Title: Acidic environment could modulate the interferon-γ expression: Implication on modulation of cancer and immune cells’ interactions

doi: 10.2478/abm-2023-0047

Figure Lengend Snippet: Altered relative expression of IFN-γ in Jurkat cells and THP-1 cells with and without stimulation with HCM at different pH values of 7.4, 6.9, and 6.5. A quantitative real-time PCR based comparison of IFN-γ produced by Jurkat cells (A) and THP-1 (B) cultured at different pH values (7.4, 6.9, and 6.5) and HCM at different pH (HCM pH 7.4, HCM pH 6.9, and HCM pH 6.5). * P < 0.05. HCM, HeLa cells conditioned medium; IFN-γ, interferon gamma.

Article Snippet: Exponentially growing HeLa cells (human cervical cancer), Jurkat cells (human T cell lymphoma), and THP-1 cells (monocytes) were procured from the National Centre for Cell Science (NCCS), India.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Comparison, Produced, Cell Culture

Western blot analysis of subcellular localization of NF-κB and expression of p38 MAPK in THP-1 (A) and Jurkat (B) cells cultured in HCM at the various pH values of 7.4, 6.9, and 6.5 for 24 h. Total cell lysate or cell lysate after fractionation (nuclear fraction and cytoplasmic fraction) were resolved on 10% SDS-PAGE for immunoblot analysis of NF-κB and p38 MAPK. β-actin was taken as an internal control. The data represent 1 of 3 similar experiments. HCM, HeLa cells conditioned medium.

Journal: Asian Biomedicine: Research, Reviews and News

Article Title: Acidic environment could modulate the interferon-γ expression: Implication on modulation of cancer and immune cells’ interactions

doi: 10.2478/abm-2023-0047

Figure Lengend Snippet: Western blot analysis of subcellular localization of NF-κB and expression of p38 MAPK in THP-1 (A) and Jurkat (B) cells cultured in HCM at the various pH values of 7.4, 6.9, and 6.5 for 24 h. Total cell lysate or cell lysate after fractionation (nuclear fraction and cytoplasmic fraction) were resolved on 10% SDS-PAGE for immunoblot analysis of NF-κB and p38 MAPK. β-actin was taken as an internal control. The data represent 1 of 3 similar experiments. HCM, HeLa cells conditioned medium.

Article Snippet: Exponentially growing HeLa cells (human cervical cancer), Jurkat cells (human T cell lymphoma), and THP-1 cells (monocytes) were procured from the National Centre for Cell Science (NCCS), India.

Techniques: Western Blot, Expressing, Cell Culture, Fractionation, SDS Page, Control

A comparison of the cytotoxic activity of THP-1 (A) and Jurkat (B) against the HeLa cells after reverting the pH (7.4, 6.9, 6.5) to physiological pH (7.4). Cytotoxic activity was assessed by MTT assay. * P < 0.05.

Journal: Asian Biomedicine: Research, Reviews and News

Article Title: Acidic environment could modulate the interferon-γ expression: Implication on modulation of cancer and immune cells’ interactions

doi: 10.2478/abm-2023-0047

Figure Lengend Snippet: A comparison of the cytotoxic activity of THP-1 (A) and Jurkat (B) against the HeLa cells after reverting the pH (7.4, 6.9, 6.5) to physiological pH (7.4). Cytotoxic activity was assessed by MTT assay. * P < 0.05.

Article Snippet: Exponentially growing HeLa cells (human cervical cancer), Jurkat cells (human T cell lymphoma), and THP-1 cells (monocytes) were procured from the National Centre for Cell Science (NCCS), India.

Techniques: Comparison, Activity Assay, MTT Assay